Review



nbp1  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Novus Biologicals nbp1
    Nbp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/SUCNR1%2FGPR91+Antibody+-+BSA+Free/pm41746808-232-70-67
    Average 94 stars, based on 22 article reviews
    nbp1 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Staining:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Control:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Immunofluorescence:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Flow Cytometry:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    RNA Expression:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Inhibition:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Fluorescence:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Knockdown:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Concentration Assay:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Western Blot:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    In Vitro:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Derivative Assay:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Cell Culture:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Two Tailed Test:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .

    Activation Assay:

    Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization
    Article Snippet: Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).Antibody specific for MMP2 (1:500, #GB11130-100) was purchased from Servicebio (Wuhan, China).. Antibody specific for SUCNR1 (1:1000, #NBP1-00861) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Article Title: Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis
    Article Snippet: murine sRANK ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were from PeproTech (Rocky Hill, NJ, USA). .. Antibodies against p50, p65, β actin, DAPI (4′,6-diamidino-2-phenylindole) and Histone H3 were purchased from Cell Signalling (Danvers, MA, USA), SUCNR1 was from Novus Biologicals (Littleton, CO, USA). .. The SUCNR1 antagonist 4c (4c) was synthesized by Shenyang Wuhe BioTech Co. (Liaoning, China) based on the previous study .



    Similar Products

    sucnr1/gpr91 antibody - bsa free  (Bio-Techne corporation)
    94
    Bio-Techne corporation sucnr1/gpr91 antibody - bsa free
    Sucnr1/Gpr91 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/SUCNR1%2FGPR91+Antibody+-+BSA+Free/custom%40nbp1-00861%4010%2E5415%2Fapallergy%2E0000000000000301
    Average 94 stars, based on 1 article reviews
    sucnr1/gpr91 antibody - bsa free - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    sucnr1 antagonist nf 56 ej40  (MedChemExpress)
    94
    MedChemExpress sucnr1 antagonist nf 56 ej40
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    Sucnr1 Antagonist Nf 56 Ej40, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/NF-56-EJ40/pmc13262611-285-7-12
    Average 94 stars, based on 1 article reviews
    sucnr1 antagonist nf 56 ej40 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    gene exp sucnr1 mm02620543 s1  (Thermo Fisher)
    94
    Thermo Fisher gene exp sucnr1 mm02620543 s1
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    Gene Exp Sucnr1 Mm02620543 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/Gene+Exp%2E+Sucnr1%2C+Mm02620543_s1/pm41897343-95-31-32
    Average 94 stars, based on 1 article reviews
    gene exp sucnr1 mm02620543 s1 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    sucnr1 ko sucnr1 mice male c57bl 6j mice  (Jackson Laboratory)
    86
    Jackson Laboratory sucnr1 ko sucnr1 mice male c57bl 6j mice
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    Sucnr1 Ko Sucnr1 Mice Male C57bl 6j Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/6j+c57bl+ko+male+mice+mice+sucnr1+sucnr1/pm42284407-207-10-21
    Average 86 stars, based on 1 article reviews
    sucnr1 ko sucnr1 mice male c57bl 6j mice - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    nbp1  (Novus Biologicals)
    94
    Novus Biologicals nbp1
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    Nbp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/SUCNR1%2FGPR91+Antibody+-+BSA+Free/pm41746808-232-70-67
    Average 94 stars, based on 1 article reviews
    nbp1 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    376951 anti sucnr1 novus biologicals  (Novus Biologicals)
    94
    Novus Biologicals 376951 anti sucnr1 novus biologicals
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    376951 Anti Sucnr1 Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/SUCNR1%2FGPR91+Antibody+-+BSA+Free/pm41746808-232-65-67
    Average 94 stars, based on 1 article reviews
    376951 anti sucnr1 novus biologicals - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    anti g protein coupled receptor 91 antibody  (Novus Biologicals)
    94
    Novus Biologicals anti g protein coupled receptor 91 antibody
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    Anti G Protein Coupled Receptor 91 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/SUCNR1%2FGPR91+Antibody+-+BSA+Free/pm41558297-56-12-8
    Average 94 stars, based on 1 article reviews
    anti g protein coupled receptor 91 antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    anti sucnr1 gpr91 antibody  (Novus Biologicals)
    94
    Novus Biologicals anti sucnr1 gpr91 antibody
    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). <t>Sucnr1</t> mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.
    Anti Sucnr1 Gpr91 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucnr1/SUCNR1%2FGPR91+Antibody+-+BSA+Free/pm41612403-66-51-57
    Average 94 stars, based on 1 article reviews
    anti sucnr1 gpr91 antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). Sucnr1 mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.

    Journal: Science Advances

    Article Title: SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes

    doi: 10.1126/sciadv.aec8873

    Figure Lengend Snippet: Plasma succinate in 8- to 12-week-old WT mice in overnight (o/n) fasting conditions or o/n fasting +2 hours (h) refed (RF) ( n = 3 to 4) ( A ). Succinate in the livers of mice subjected to o/n fasting or o/n fasting +2 hours RF ( n = 3 to 4) ( B ). Sucnr1 mRNA expression in livers of 8- to 12-week-old WT mice ad libitum–fed, fasted for 24 hours, or fasted for 24 + 24 hours RF ( n = 5 to 6) ( C ) and comparison between mice fed ad libitum or subjected to a caloric restriction ( n = 5 to 4) ( D ). Sucnr1 expression in murine livers extracted from transcriptomic data from two independent studies ( , ) comparing 24-hour fasting versus 24-hour fasting +2-hour RF (left bar) and 16-hour fasting versus control (right bar) ( E ). Succinate concentration in the conditioned medium of AML12 after 24-hour treatment of 0, 5, or 25 mM glucose, as well as 10 mM 2-deoxy- d -glucose (2DG) ( n = 6) ( F ) or 2 mM glutamine ( n = 3) ( G ). Fractional enrichment of succinate after administration of 13 C-glucose or 13 C-glutamine in primary hepatocytes of Sucnr1 fl/fl mice ( n = 3) ( H ). Sucnr1 mRNA expression after 24 hours of 0, 5, or 25 mM glucose treatment ( n = 4 to 6) ( I ). Results are presented as means ± SEM [(A), (B), (C), (D), (F), (G), and (H)] or as fold change (fasted versus RF/fed) (E); * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; a, P < 0.05 versus 0 mM glucose w/o 2DG; b, P < 0.05 versus 5.5 mM glucose w/o 2DG; c, P < 0.05 versus 25 mM glucose w/o 2DG [two-tailed unpaired t test [(A), (B), (D), (G), and (H)]; one-way analysis of variance (ANOVA) plus Tukey’s multiple comparisons test [(C) and (I)]; one-way ANOVA plus Šídák’s multiple comparisons test (F). a.u., arbitrary units.

    Article Snippet: THLE-2 cells were treated with 1 μM SUCNR1 antagonist NF-56-EJ40 ( ) (MedChemExpress) for 5 hours or with the same treatment but with the addition of 100 μM SUCNR1 agonist cis -epoxysuccinic acid ( ) (E0449, Tokyo Chemical Industry) for the last 3 hours of treatment in the BEBM medium without added supplements or FBS.

    Techniques: Clinical Proteomics, Expressing, Comparison, Control, Concentration Assay, Two Tailed Test

    Reverse transcription quantitative polymerase chain reaction (RT-qPCR) of Sucnr1 in livers and hepatocytes from 10-week-old Hep- Sucnr1 KO mice compared to controls ( n = 7 to 7; 6 to 7) ( A ). Body weight ( n = 7 to 7) ( B ). Plasma lipid profile ( n = 3 to 3) ( C ). Glucose tolerance test and area under the curve ( n = 6 to 3) ( D ). ITT and area under the curve ( n = 9 to 5) ( E ). PTT and area under the curve ( n = 9 to 9) ( F ). Glucose concentration in plasma after an o/n fasting ( n = 6 to 5) ( G ). Glucose ( H ) and ketones ( I ) concentration in plasma in response to fasting and area under the curve ( n = 3 to 8). Amino acids in plasma after an o/n fasting ( n = 3 to 3) ( J ). Glucagon response in plasma to an o/n fasting ( n = 3 to 4) ( K ). Glucose in the medium of primary hepatocytes isolated from Sucnr1 fl/fl mice and Hep- Sucnr1 KO mice subjected to a medium without glucose supplemented with gluconeogenic precursors ( n = 3 to 3) ( L ). Results are presented as means ± SEM or as log 2 fold change Hep- Sucnr1 KO versus Sucnr1 fl/fl mice (L) and statistical significance studied by two-tailed unpaired t test [(A), (C), (J), (K), and (L), bar graphs]; Mann-Whitney test [(B), (C), (G), and (J)]; two-way ANOVA [(D) to (F)] or mixed-effects model [(H) and (I)]; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. AABA, α-aminobutyric acid; AIBA, α-aminoisobutyric acid; GABA, γ-aminobutyric acid; HDL, high-density lipoprotein; NEFA, nonesterified fatty acids; TGs, triglycerides.

    Journal: Science Advances

    Article Title: SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes

    doi: 10.1126/sciadv.aec8873

    Figure Lengend Snippet: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) of Sucnr1 in livers and hepatocytes from 10-week-old Hep- Sucnr1 KO mice compared to controls ( n = 7 to 7; 6 to 7) ( A ). Body weight ( n = 7 to 7) ( B ). Plasma lipid profile ( n = 3 to 3) ( C ). Glucose tolerance test and area under the curve ( n = 6 to 3) ( D ). ITT and area under the curve ( n = 9 to 5) ( E ). PTT and area under the curve ( n = 9 to 9) ( F ). Glucose concentration in plasma after an o/n fasting ( n = 6 to 5) ( G ). Glucose ( H ) and ketones ( I ) concentration in plasma in response to fasting and area under the curve ( n = 3 to 8). Amino acids in plasma after an o/n fasting ( n = 3 to 3) ( J ). Glucagon response in plasma to an o/n fasting ( n = 3 to 4) ( K ). Glucose in the medium of primary hepatocytes isolated from Sucnr1 fl/fl mice and Hep- Sucnr1 KO mice subjected to a medium without glucose supplemented with gluconeogenic precursors ( n = 3 to 3) ( L ). Results are presented as means ± SEM or as log 2 fold change Hep- Sucnr1 KO versus Sucnr1 fl/fl mice (L) and statistical significance studied by two-tailed unpaired t test [(A), (C), (J), (K), and (L), bar graphs]; Mann-Whitney test [(B), (C), (G), and (J)]; two-way ANOVA [(D) to (F)] or mixed-effects model [(H) and (I)]; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. AABA, α-aminobutyric acid; AIBA, α-aminoisobutyric acid; GABA, γ-aminobutyric acid; HDL, high-density lipoprotein; NEFA, nonesterified fatty acids; TGs, triglycerides.

    Article Snippet: THLE-2 cells were treated with 1 μM SUCNR1 antagonist NF-56-EJ40 ( ) (MedChemExpress) for 5 hours or with the same treatment but with the addition of 100 μM SUCNR1 agonist cis -epoxysuccinic acid ( ) (E0449, Tokyo Chemical Industry) for the last 3 hours of treatment in the BEBM medium without added supplements or FBS.

    Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Clinical Proteomics, Concentration Assay, Isolation, Two Tailed Test, MANN-WHITNEY

    Normalized abundance ( A ) and fractional enrichment ( B ) of glycolytic, gluconeogenic, and TCA or Krebs cycle intermediates in response to 13 C-glutamine administration to Sucnr1 fl/fl and Hep- Sucnr1 KO hepatocytes for 1 hour ( n = 3 to 3). Results are presented as means ± SEM and statistical significance studied by two-tailed unpaired t test or Mann-Whitney test; * P < 0.05; ** P < 0.01; *** P < 0.001; green, up-regulated; red, down-regulated; yellow, suspected carbon flow. DHAP, dihydroxyacetone phosphate; glucose-6-P, glucose-6-phosphate; PEP, phosphoenolpyruvate; ribose-5-P, ribose-5-phosphate; 2-PG, 2-phosphoglycerate; 3-PG, 3-phosphoglycerate. Created in BioRender. Fernández-Veledo, S. (2026) https://BioRender.com/6yx74jq .

    Journal: Science Advances

    Article Title: SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes

    doi: 10.1126/sciadv.aec8873

    Figure Lengend Snippet: Normalized abundance ( A ) and fractional enrichment ( B ) of glycolytic, gluconeogenic, and TCA or Krebs cycle intermediates in response to 13 C-glutamine administration to Sucnr1 fl/fl and Hep- Sucnr1 KO hepatocytes for 1 hour ( n = 3 to 3). Results are presented as means ± SEM and statistical significance studied by two-tailed unpaired t test or Mann-Whitney test; * P < 0.05; ** P < 0.01; *** P < 0.001; green, up-regulated; red, down-regulated; yellow, suspected carbon flow. DHAP, dihydroxyacetone phosphate; glucose-6-P, glucose-6-phosphate; PEP, phosphoenolpyruvate; ribose-5-P, ribose-5-phosphate; 2-PG, 2-phosphoglycerate; 3-PG, 3-phosphoglycerate. Created in BioRender. Fernández-Veledo, S. (2026) https://BioRender.com/6yx74jq .

    Article Snippet: THLE-2 cells were treated with 1 μM SUCNR1 antagonist NF-56-EJ40 ( ) (MedChemExpress) for 5 hours or with the same treatment but with the addition of 100 μM SUCNR1 agonist cis -epoxysuccinic acid ( ) (E0449, Tokyo Chemical Industry) for the last 3 hours of treatment in the BEBM medium without added supplements or FBS.

    Techniques: Two Tailed Test, MANN-WHITNEY

    OCR, basal and maximal respiration, ATP production, spare respiratory capacity, proton leak, and nonmitochondrial O 2 consumption in primary hepatocytes from WT and Sucnr1 −/− mice; substrates: 10 mM glucose, 2 mM l -glutamine, 1 mM sodium pyruvate ( n = 4 to 4) ( A ). ECAR under the same conditions ( n = 4 to 4) ( B ). OCR parameters [as in (A)] and ECAR in primary hepatocytes isolated from Sucnr1 fl/fl and Hep- Sucnr1 KO mice ( n = 3 to 3) ( C and D ). OCR, basal respiration, phosphorylating respiration, proton leak, ATP production, maximal respiration, and nonmitochondrial O 2 consumption in isolated mitochondria from Sucnr1 fl/fl and Hep- Sucnr1 KO livers; substrates: 10 mM succinate, 2 μM rotenone ( n = 3 to 3) ( E ). Transmission electron microscopy images of Sucnr1 fl/fl and Hep- Sucnr1 KO livers ( F ). Area, perimeter, circularity, and number of mitochondria in the liver ( n = 4 to 4) ( G ). Western blot of total OXPHOS complexes in liver extracts ( n = 4 to 4) ( H ). Volcano plot of liver proteomics: Hep- Sucnr1 KO versus Sucnr1 fl/fl ; labeled gene symbols denote mitochondrial proteins with P < 0.05 and −0.58 < log 2 fold change <0.58 ( n = 3 to 3) ( I ). Significantly enriched GO cellular component and Wiki pathways between genotypes ( n = 3 to 3) ( J ). Immunoblot of ATF6, ATF4, and CHOP in o/n fasted (fasted) and o/n fasted plus 2 hours of refeeding (RF) livers, with densitometry normalized to β-actin ( n = 7 to 7) ( K ). Plasma AST and ALT ( n = 8 to 7) ( L ). Results are presented as means ± SEM; statistical analyses: two-tailed unpaired t test [(A), (B), (C), (D), (E), (H), and (K)] or Mann-Whitney test [(G), (H), (K), and (L)]. Proteomics are shown as log 2 fold changes with limma’s empirical Bayes moderated t statistics (I). Pathway enrichment is presented as running enrichment scores (ES) across the ranked gene list (J); * P < 0.05; ** P < 0.01; and **** P < 0.0001. ALT, alanine transaminase; AST, aspartate transaminase; ATF4, activating transcription factor 4; ATF6, activating transcription factor 6; C, complex; CHOP, C/EBP homologous protein.

    Journal: Science Advances

    Article Title: SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes

    doi: 10.1126/sciadv.aec8873

    Figure Lengend Snippet: OCR, basal and maximal respiration, ATP production, spare respiratory capacity, proton leak, and nonmitochondrial O 2 consumption in primary hepatocytes from WT and Sucnr1 −/− mice; substrates: 10 mM glucose, 2 mM l -glutamine, 1 mM sodium pyruvate ( n = 4 to 4) ( A ). ECAR under the same conditions ( n = 4 to 4) ( B ). OCR parameters [as in (A)] and ECAR in primary hepatocytes isolated from Sucnr1 fl/fl and Hep- Sucnr1 KO mice ( n = 3 to 3) ( C and D ). OCR, basal respiration, phosphorylating respiration, proton leak, ATP production, maximal respiration, and nonmitochondrial O 2 consumption in isolated mitochondria from Sucnr1 fl/fl and Hep- Sucnr1 KO livers; substrates: 10 mM succinate, 2 μM rotenone ( n = 3 to 3) ( E ). Transmission electron microscopy images of Sucnr1 fl/fl and Hep- Sucnr1 KO livers ( F ). Area, perimeter, circularity, and number of mitochondria in the liver ( n = 4 to 4) ( G ). Western blot of total OXPHOS complexes in liver extracts ( n = 4 to 4) ( H ). Volcano plot of liver proteomics: Hep- Sucnr1 KO versus Sucnr1 fl/fl ; labeled gene symbols denote mitochondrial proteins with P < 0.05 and −0.58 < log 2 fold change <0.58 ( n = 3 to 3) ( I ). Significantly enriched GO cellular component and Wiki pathways between genotypes ( n = 3 to 3) ( J ). Immunoblot of ATF6, ATF4, and CHOP in o/n fasted (fasted) and o/n fasted plus 2 hours of refeeding (RF) livers, with densitometry normalized to β-actin ( n = 7 to 7) ( K ). Plasma AST and ALT ( n = 8 to 7) ( L ). Results are presented as means ± SEM; statistical analyses: two-tailed unpaired t test [(A), (B), (C), (D), (E), (H), and (K)] or Mann-Whitney test [(G), (H), (K), and (L)]. Proteomics are shown as log 2 fold changes with limma’s empirical Bayes moderated t statistics (I). Pathway enrichment is presented as running enrichment scores (ES) across the ranked gene list (J); * P < 0.05; ** P < 0.01; and **** P < 0.0001. ALT, alanine transaminase; AST, aspartate transaminase; ATF4, activating transcription factor 4; ATF6, activating transcription factor 6; C, complex; CHOP, C/EBP homologous protein.

    Article Snippet: THLE-2 cells were treated with 1 μM SUCNR1 antagonist NF-56-EJ40 ( ) (MedChemExpress) for 5 hours or with the same treatment but with the addition of 100 μM SUCNR1 agonist cis -epoxysuccinic acid ( ) (E0449, Tokyo Chemical Industry) for the last 3 hours of treatment in the BEBM medium without added supplements or FBS.

    Techniques: Isolation, Transmission Assay, Electron Microscopy, Western Blot, Labeling, Clinical Proteomics, Two Tailed Test, MANN-WHITNEY

    Twelve-week-old Sucnr1 fl/fl and Hep- Sucnr1 KO mice were subjected to o/n fasting (fasted) or o/n fasting +2 hours of refeeding (RF), and the livers and plasma were collected for analysis. Volcano plot illustrating the differential protein expression between RF and fasting in Sucnr1 fl/fl (left) and Hep- Sucnr1 KO (right) mice with P value <0.05 and −0.58 < log 2 fold change <0.58 ( n = 3 to 4) ( A ). Log 2 fold change (LFC) RF versus fasting in Sucnr1 fl/fl and Hep- Sucnr1 KO of the top 20 most significantly different proteins in Sucnr1 fl/fl mice ( n = 4 to 4) ( B ). GSEA for GO Biological Process, GO Cellular Component, and Reactome pathways in Sucnr1 fl/fl (top) and Hep- Sucnr1 KO (bottom) mice ( n = 3 to 4) ( C ). Western blot analysis of phosphorylated mTOR (p-mTOR) and total mTOR, with densitometric quantification of the p-mTOR/total mTOR ratio in livers of Sucnr1 fl/fl and Hep- Sucnr1 KO mice in Fasted ( n = 3) and RF ( n = 4) conditions, normalized to β actin ( D ). Fold change of glycogen content RF versus fasting in the livers of Sucnr1 fl/fl and Hep- Sucnr1 KO mice ( n = 4 to 4) ( E ). Fold change of plasma amino acids RF versus fasting in Sucnr1 fl/fl and Hep- Sucnr1 KO mice ( n = 3 to 4) ( F ). Results are presented as log 2 fold changes with limma’s empirical Bayes moderated t statistics [(A) and (B)] or as running enrichment score (ES) across the ranked gene list, with vertical bars marking the positions of pathway members (C). Results are shown as means ± SEM and statistical significance studied by two-tailed unpaired t test [(D) to (F)] or Mann-Whitney test [(D) and (F)]; * P < 0.05; ** P < 0.01; *** P < 0.001. GABA, γ-aminobutyric acid; AIBA, α-aminoisobutyric acid; AABA, α-aminobutyric acid.

    Journal: Science Advances

    Article Title: SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes

    doi: 10.1126/sciadv.aec8873

    Figure Lengend Snippet: Twelve-week-old Sucnr1 fl/fl and Hep- Sucnr1 KO mice were subjected to o/n fasting (fasted) or o/n fasting +2 hours of refeeding (RF), and the livers and plasma were collected for analysis. Volcano plot illustrating the differential protein expression between RF and fasting in Sucnr1 fl/fl (left) and Hep- Sucnr1 KO (right) mice with P value <0.05 and −0.58 < log 2 fold change <0.58 ( n = 3 to 4) ( A ). Log 2 fold change (LFC) RF versus fasting in Sucnr1 fl/fl and Hep- Sucnr1 KO of the top 20 most significantly different proteins in Sucnr1 fl/fl mice ( n = 4 to 4) ( B ). GSEA for GO Biological Process, GO Cellular Component, and Reactome pathways in Sucnr1 fl/fl (top) and Hep- Sucnr1 KO (bottom) mice ( n = 3 to 4) ( C ). Western blot analysis of phosphorylated mTOR (p-mTOR) and total mTOR, with densitometric quantification of the p-mTOR/total mTOR ratio in livers of Sucnr1 fl/fl and Hep- Sucnr1 KO mice in Fasted ( n = 3) and RF ( n = 4) conditions, normalized to β actin ( D ). Fold change of glycogen content RF versus fasting in the livers of Sucnr1 fl/fl and Hep- Sucnr1 KO mice ( n = 4 to 4) ( E ). Fold change of plasma amino acids RF versus fasting in Sucnr1 fl/fl and Hep- Sucnr1 KO mice ( n = 3 to 4) ( F ). Results are presented as log 2 fold changes with limma’s empirical Bayes moderated t statistics [(A) and (B)] or as running enrichment score (ES) across the ranked gene list, with vertical bars marking the positions of pathway members (C). Results are shown as means ± SEM and statistical significance studied by two-tailed unpaired t test [(D) to (F)] or Mann-Whitney test [(D) and (F)]; * P < 0.05; ** P < 0.01; *** P < 0.001. GABA, γ-aminobutyric acid; AIBA, α-aminoisobutyric acid; AABA, α-aminobutyric acid.

    Article Snippet: THLE-2 cells were treated with 1 μM SUCNR1 antagonist NF-56-EJ40 ( ) (MedChemExpress) for 5 hours or with the same treatment but with the addition of 100 μM SUCNR1 agonist cis -epoxysuccinic acid ( ) (E0449, Tokyo Chemical Industry) for the last 3 hours of treatment in the BEBM medium without added supplements or FBS.

    Techniques: Clinical Proteomics, Expressing, Western Blot, Two Tailed Test, MANN-WHITNEY

    SUCNR1 immunohistochemistry in adult WT mouse liver ( A ). Sucnr1 expression (transcripts per million, TPM) in CD146 magnetic bead–prepurified LECs isolated from 30 C57BL/6 mice by fluorescence-activated cell sorting with c-kit antibody to separate four zones based on the gradient of expression along the porto-central axis of this protein : portal node (PN), periportal zone (PP), pericentral zone (PC), and central vein (CV) [data from table S1 in reference ] ( B ). Sucnr1 expression [fraction of total unique molecular identifiers (UMIs)] in paired hepatocytes-LECs, isolated from three C57BL/6 mice assigned to layers one to eight according to zonation probability based on the expression of 21 pericentral and 30 periportal genes [data from table S5 in reference ] ( C ). RT-qPCR of Sucnr1 mRNA in WT mouse liver at E19.5, P5, P10, and P14 (P5 to P14), and 3 months of age ( n = 4 to 5) ( D ). GSEA of pericentral, periportal, and Wnt pathway gene signatures from hepatocytes’ transcriptomics data (Hep- Sucnr1 KO mice versus Sucnr1 fl/fl mice) ( n = 3 to 3) ( E ). Chord diagram of gene ontologies overrepresentation analysis in pericentral, periportal, and Wnt gene sets ( n = 3 to 3) ( F ). Immunohistochemistry for glutamine synthetase (GLUL) and cytochrome P450 2F2 (CYP2F2) in Sucnr1 fl/fl and Hep- Sucnr1 KO livers with quantification of mean optical density and stained area ( n = 3 to 3) ( G ). Results are presented as means ± SEM; one-way ANOVA with Tukey’s multiple comparisons test ( P < 0.05 indicated by different letters) [(B) to (D)]. GSEA results are shown as running enrichment scores (ES) across the ranked gene list (E). Results are shown as chord diagrams, with connection widths proportional to the absolute log 2 fold change of each gene (F). Results are presented as means ± SEM and statistical significance studied by two-tailed unpaired t test or Mann-Whitney test (G); ** P < 0.01.

    Journal: Science Advances

    Article Title: SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes

    doi: 10.1126/sciadv.aec8873

    Figure Lengend Snippet: SUCNR1 immunohistochemistry in adult WT mouse liver ( A ). Sucnr1 expression (transcripts per million, TPM) in CD146 magnetic bead–prepurified LECs isolated from 30 C57BL/6 mice by fluorescence-activated cell sorting with c-kit antibody to separate four zones based on the gradient of expression along the porto-central axis of this protein : portal node (PN), periportal zone (PP), pericentral zone (PC), and central vein (CV) [data from table S1 in reference ] ( B ). Sucnr1 expression [fraction of total unique molecular identifiers (UMIs)] in paired hepatocytes-LECs, isolated from three C57BL/6 mice assigned to layers one to eight according to zonation probability based on the expression of 21 pericentral and 30 periportal genes [data from table S5 in reference ] ( C ). RT-qPCR of Sucnr1 mRNA in WT mouse liver at E19.5, P5, P10, and P14 (P5 to P14), and 3 months of age ( n = 4 to 5) ( D ). GSEA of pericentral, periportal, and Wnt pathway gene signatures from hepatocytes’ transcriptomics data (Hep- Sucnr1 KO mice versus Sucnr1 fl/fl mice) ( n = 3 to 3) ( E ). Chord diagram of gene ontologies overrepresentation analysis in pericentral, periportal, and Wnt gene sets ( n = 3 to 3) ( F ). Immunohistochemistry for glutamine synthetase (GLUL) and cytochrome P450 2F2 (CYP2F2) in Sucnr1 fl/fl and Hep- Sucnr1 KO livers with quantification of mean optical density and stained area ( n = 3 to 3) ( G ). Results are presented as means ± SEM; one-way ANOVA with Tukey’s multiple comparisons test ( P < 0.05 indicated by different letters) [(B) to (D)]. GSEA results are shown as running enrichment scores (ES) across the ranked gene list (E). Results are shown as chord diagrams, with connection widths proportional to the absolute log 2 fold change of each gene (F). Results are presented as means ± SEM and statistical significance studied by two-tailed unpaired t test or Mann-Whitney test (G); ** P < 0.01.

    Article Snippet: THLE-2 cells were treated with 1 μM SUCNR1 antagonist NF-56-EJ40 ( ) (MedChemExpress) for 5 hours or with the same treatment but with the addition of 100 μM SUCNR1 agonist cis -epoxysuccinic acid ( ) (E0449, Tokyo Chemical Industry) for the last 3 hours of treatment in the BEBM medium without added supplements or FBS.

    Techniques: Immunohistochemistry, Expressing, Isolation, Fluorescence, FACS, Quantitative RT-PCR, Transcriptomics, Staining, Two Tailed Test, MANN-WHITNEY